• Study of Glycine and Alanine Coupled with Carboxylic Acids as Biofilm Inhibitors in Common Bacteria Strains

      Goode, David; Hensel, Linda; Severiano, Sara; Tondreau, Jacob; Kight, Parker (2021)
      ?Biofilm is a substance secreted by bacteria cells that offers protection to the bacteria colony, typically from host immune cells. Scientists have found biofilm to cause complications and infections in medical devices such as pacemakers and catheters. Biofilm production is stimulated when enough bacteria communicate through a process known as quorum sensing. This study aimed to identify possible amide coupled carboxylic acids and amino acids that resemble quorum-sensing signaling molecules as biofilm inhibitors in Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, and Bacillus subtilis. Crystal violet assays were used to test for biofilm inhibition; disk diffusion, congo red, and use-dilution assay; and planktonic assays were conducted to test for traditional antibiotic properties of bactericidal or bacteriostatic activity. Gly-4 and Gly-32 were found to be biofilm inhibitors in S. mutans, with 53% and 47% biofilm inhibition respectively; neither drug showed signs of traditional antibacterial properties. Future experiments are required to corroborate this study�s findings, and to explore the efficacy of other drugs with similar functional groups to develop more sophisticated biofilm inhibitors.